The ten cell types considered in the deconvolution of the tissue were Astrocytes (Astro), Endothelial (Endo), Macrophage (Macro), Microglia (Micro), Mural cells, Oligodendrocytes (Oligo), Oligodendrocyte Progenitor Cells (OPC), T cells, Excitatory Neurons (Excit), and Inhibitory Neurons (Inhib). (10.1101/2020.39) which includes 70,615 high-quality nuclei across five human brain regions: nucleus accumbens (NAc), amygdala (AMY), subgenual anterior cingulate cortex (sACC), hippocampus (HPC), and dorsolateral prefrontal cortex (DLPFC). Deconvolution seeks to estimate the composition of cell types in the sequenced tissue to examine biological differences between samples, or minimize confounding technical variables, such as differences in dissection, in downstream analysis.įor the reference single nucleus RNA-seq dataset we utilized the data set from Tran et al. Bulk RNA-seq from tissue homogenates obscures this complexity. The plaintiff said he had submitted all interview materials and notes in his possession.Lieber Institute for Brain Development, Baltimore, Maryland, United Statesīackground: The brain contains a wide variety of structures, that in turn are made up of many different cell types with individual complex functions. The next day, Wymbs told Woodberry he believed his interview notes regarding the volleyball coach position were insufficient, according to court documents. The student’s name was reportedly never used. Quick told him the student had made up the missed classes and the failure-to-attend status had been changed. Regarding the reporter’s question about grade changes, Woodberry said a softball player had been ineligible because of a failure to attend classes, the lawsuit states. The plaintiff reportedly denied the interview was mishandled and identified the candidates interviewed for the job. Woodberry was contacted by a member of the local media about accusations that Quick changed grades to keep athletes eligible and had mishandled the interview process when she hired the volleyball coach, court documents state. On March 18, another assistant principal, Randy Jackson, emailed Woodberry about a media request and told him that all information provided needed to be “truthful with 100% transparency.” Any and all false or misconstrued information would be “looked into” by the district, according to the lawsuit. The plaintiff protested because he didn’t believe the two had done anything to warrant being fired and was then told to tell them the programs “needed to go in a different direction,” the lawsuit states. On March 1, Wymbs and Frasier told Woodberry to fire volleyball coach Hillary Pratt and basketball coach Pete Ellis, according to court documents. When the plaintiff said he believed he could do both jobs, the lawsuit claims Wymbs said, “I will get your a-, you (expletive), and “You will listen to a white woman but not a black man, f- you.” 16, 2018, Woodberry said Wymbs and then-assistant principal Mark Frasier called him multiple times while he was with his son on a college baseball visit in Charleston to pressure him to resign as football coach. Woodberry said he then tried to reach Wymbs but was unsuccessful.īetween Feb. 16 to choose which position he wanted to keep – athletic director or head football coach. 14, 2018 from the school’s assistant principal who gave him a deadline of Feb. Woodberry said he received a call on Feb. Wymbs, who was named interim principal for West Florence High in early 2018 following the resignation of former principal Pam Quick, encouraged Woodberry to reduce his duties to athletic director only after the plaintiff said he had no plans to leave his coaching position, the suit stated.
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